Previous Seminars
ICaMB Seminar - Stephan Uphoff
- Venue: Dental Lecture Theatre D
- Start: Wed, 13 Feb 2019 13:00:00 GMT
- End: Wed, 13 Feb 2019 14:00:00 GMT
Stephan Uphoff's group investigates DNA repair and mutagenesis in bacteria. They seek to understand the mechanisms of DNA repair pathways and how mutation rates are regulated through DNA damage responses. Their goal is to directly measure these fundamental processes inside individual living cells. To this end, they develop single-molecule/super-resolution microscopy, single-cell manipulation, microfluidics, flow cytometry, and whole genome sequencing techniques. They also advance data analysis and computational modeling for quantitative descriptions of DNA repair and mutagenesis. They are currently very interested in the consequences of gene expression noise on the fidelity of genome maintenance. This work also informs about the origins of mutations that lead to antibiotic resistance.
Publications
- Scc2/Nipbl Hops Between Chromosomal Cohesin Rings After Loading
Rhodes J, Mazza D, Nasmyth K, Uphoff S
eLife e30000 (2017)
- Single-molecule analysis of bacterial DNA repair and mutagenesis
Uphoff S, Sherratt DJ
Annual Reviews of Biophysics, 46, 411-432 (2017)
- Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli
Stracy M, Jaciuk M, Uphoff S, Kapanidis AN, Nowotny M, Sherratt DJ, Zawadzki P
Nature Communications, 7, 12568 (2016)
- Stochastic activation of a DNA damage response causes cell-to-cell mutation rate variation
Uphoff S, Lord ND, Potvin-Trottier L, Okumus B, Sherratt DJ, Paulsson J
Science, 27290, 1094-1097 (2016)
- Studying the organization of DNA repair by single-cell and single-molecule imaging
Uphoff S, Kapanidis AN
DNA Repair, 20, 32-40 (2014)
- Single-molecule DNA repair in live bacteria
Uphoff S, Reyes-Lamothe R, Garza de Leon F, Sherratt DJ, Kapanidis AN
Proc Natl Acad Sci U S A , 110(20), 8063-8068 (2013)
- In vivo architecture and action of bacterial structural maintenance of chromosome proteins
Badrinarayanan A, Reyes-Lamothe R, Uphoff S, Leake MC, Sherratt DJ
Science, 338(6106), 528-531 (2012)
- DAOSTORM: an algorithm for high- density super-resolution microscopy
Holden SJ, Uphoff S, Kapanidis AN
Nature Methods, 8(4), 279-280 (2011)
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