Previous Seminars
ICaMB seminar - Prof Francis Barr
- Venue: Baddiley-Clark Seminar Room
- Start: Wed, 25 Nov 2015 13:00:00 GMT
- End: Wed, 25 Nov 2015 14:00:00 GMT
Professor Francis Barr, visiting from the University of Oxford, will give the ICaMB seminar:
Why do eukaryotic cells choose cytokinesis on exit from mitosis?
Cells grow and then divide by binary fission. In eukaryotes, this cycle is driven by the synthesis and destruction of protein kinases. In this talk I will describe the role of PP2A family phosphatases, highlighting the crucial roles they play in the mechanism of division site selection. Specifically, we have investigated how a protein phosphatase control system, the PP2A-B55/ENSA/Greatwall (BEG) pathway, regulates the exit from mitosis and cytokinesis. This seminar will address the central unanswered problem in this area, of how specific substrates are selected for regulation by PP2A-B55. To do this we have designed and implemented a temporal phosphoproteomic analysis that allows the precise definition of phosphatase substrates. This quantitative mass spectrometry data was then incorporated into our previously validated model for the BEG pathway to give systems level view of PP2A-B55 function in mitotic exit. Our strategy allows precise comparison of different substrate dephosphorylation kinetics at a global level, and means we can understand how BEG pathway substrates are selected. During the seminar I will introduce these unpublished findings and explain how they link two major pathways regulating eukaryotic cell division.