2014 participants
Chileleko Siachisumo
The progression through mitosis is meticulously coordinated to ensure the fidelity separation of its duplicated genomes into two daughter cells, this is monitored by a complex surveillance mechanism called spindle assembly checkpoint (SAC) or mitotic checkpoint. We carried out antibody staining experiments that use the Duolink in situ PLA assay technique with appropriate primary antibodies to investigate the spatial-temporal interaction profiles of cyclin B and Cdc20-cyclin B in thymidine synchronized human HeLa cells. The fluorescent signals of the interaction between the proteins of interest in fixed cells were detected using a laser confocal microscope.
Funding Source: Newcastle University