HTS - High Throughput Screening

The High Throughput Screening Facility (HTSF) enables individual research groups to use large-scale screening technologies and approaches as part of their research arsenal. The facility is located in two sites within the Medical School and also has access to robotics situated in the Institute for Genetic Medicine at the Centre for Life. Utilising an extensive suite of robotic systems and high throughput reagents HTSF staff collaborate closely with research groups throughout a project lifecycle from developing assays to screening through to final data interpretation and secondary screening.

Microbial Screening

The HTSF has access to many arrayed collections of S. cerevisiae, C. albicans and S. pombe that can be used for screens in these organisms. A unique combination of colony pinning robots, automated agar plate imaging systems and plate readers allows researchers to precisely measure the fitness of thousands of independent microbial colonies.

Mammalian Screening

The facility retains a Beckman FX liquid handling system dedicated solely to mammalian cell work. In combination with and extensive (7500) siRNA library and MRCT drug library (20000 compounds) the facility provides a comprehensive resource for mammalian cell screening. For those not interested in genome wide screening, all libraries are further segmented into functional groupings allowing researchers to screen specific areas of cellular biology.

Collaborative Approaches

Our equipment allows researchers to explore small to large-scale experiments in 96-, 384- or 1536-well format with many and various readouts. We regularly interact with other core facilities within the Faculty of Medical Sciences to develop assays and maintain research facilities at the cutting edge of Science.

Contact details

High Throughput Screening Facility
Faculty of Medical Sciences
Newcastle University
Cookson Building
The Medical School
Framlington Place
Newcastle upon Tyne
0191 2084963

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