0
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100
150
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DDC1
RAD17
RAD24
UPF3
NAM7
EXO1
CHK1
NMD2
EBS1
RAD9
HIS3
MRE11
RAD50
XRS2
TEL1
EST1
EST3
YKU80
YKU70
CDC
+
27
o
C Fitness (MDR x MDP)
cdc13-1
27
o
C Fitness (MDR x MDP)
●
MRX complex
Checkpoint clamp/loader
Nonsense mediated mRNA decay
Ku complex
Telomerase
Other gene of interest
QFA
of
telomere-defective
cdc13-1
strains.
Strains
with
cdc13-1
or
ura3∆
(CDC
+
)
mutations
were
crossed
with
300
other
gene
deletions
by
SGA
Fitness
of
the
progeny
was
assessed
by
QFA
at
27
o
C.
Each
dot,
grey
or
coloured,
indicates
the
position
of
a
single
gene
deletion
in
combination
with
other
mutations.
Gene
deletions
known
to
affect
telomere
biology
are
co-coloured.
The
neutral
his3∆
mutation
is
at
the
inte
-
section
of
the
blue
lines.
A
linear
regression
through
all
points
is
solid
grey
and
the
1:1
line
is
dashed.
The
initial
plot
was
generated
in
the
visualization
tool
in
our
R
package and relablled and coloured using Adobe Illustrator.