Arda Deniz Tugrul

• BSc (Hons) Biomedical Genetics
• Investigating the effects of cdc28 on cell cycle regulation in Saccharomyces Cerevisiae

I have investigated the effects of cdc28 protein on cell cycle regulation in Saccharomyces Cerevisiae. First, I have cultured the yeast cdc28 query strain on a YPD rich media. I have also arrayed the yeast alpha library and cultured them. After culturing both my query cdc28 strain as well as the alpha-library strain, I have crossed both strains for mating on a new plate with robot pinning technology at high-throughput screening facility. After mating event, I have selectively picked the colonies and cultured them again on a diploid selection plates, then after diploid selection, I have finally selected the spores through the use of eukaryotic selective marker (G418) on new sporulation plates to ensure that I was analysing the spores that had the library deletion as well as my query cdc28 deletion. After collecting my raw data, I have analysed my final data through SGA Tools software, looking at colony size of each strain to observe the fitness.

Funding source: Newcastle University

Project supervisor: Dr Peter Banks