Ya Min Ng

• BSc Biomedical Sciences 2+1 NUMed and Newcastle UK
• Identification of Escherichia coli antibiotic resistance genes present in surface and domestic tap water at Johor, Malaysia

Antibiotic resistance is the situation whereby microorganisms can thrive despite presence of antibiotics. Antibiotic resistance is a growing public health concern worldwide affecting not only mankind but also animals and the environment. Water samples were collected from a downstream river and domestic tap water at a small village Southwest of Johor, Malaysia. Samples were then plated on selective agar to differentiate between Escherichia coli (E. coli) and other bacteria. Gram staining and light microscopy were also performed to identify the classification and morphology of bacterial cells present in the water samples. Antibiotic resistance of E. coli was detected through disc diffusion method on Müeller-Hinton (MH) agar. Diameter of zone of complete inhibition was measured and compared with Clinical and Laboratory Standards Institute guideline for antibiotic resistance which shown surprising results. DNA extraction was done to allow for future DNA sequencing to identify the type of antibiotic resistance genes (ARGs) present.

Funding source: NUMed Malaysia

Supervisor: Dr Mukhrizah Othman