Alice Bradbury

• BSc (Hons) Pharmacology
• Characterisation of the interaction between CDK2/cyclin E and CINP and its role in regulating replication initiation complex formation

A cell completes one round of the cell cycle when it grows and divides to generate two daughter cells. A family of proteins called cyclin-dependent kinases (CDKs) control this process.  CDK2 bound to cyclin E reversibly modifies a number of substrates that are required to ensure that DNA replication in initiated appropriately.  CDK2-interacting protein (CINP) has been reported to bind to CDK2/cyclin E at DNA origins.  The aim of this project is to characterise the CDK2/cyclin E-CINP interaction using purified proteins to further understand the regulation of CDK2/cyclin E activity during the initiation of DNA replication.

Project Supervisor: Prof Martin Noble, Northern Institute for Cancer Research

Funding Source: Newcastle University