Techniques

Microscope

Molecular Techniques

  • Protein extraction, preparation and purification:
    • Immunoprecipitation
    • SDS-PAGE
    • Ultrafiltration
    • LC/Ion-exchange chromatography
  • Nucleic acid extraction, preparation and purification:
    • PCR
    • qPCR
    • Gel Electrophoresis
  • Diagnostics:
    • Western Blotting
    • In situ hybridisation

Adhesion Measurement

  • Flow Cell
  • Water Jet
  • Mechanical Testing

Behavioural Analysis

  • Tracking in Two Dimensions
  • Tracking in Three Dimensions

Imaging

  • Light Microscopy: Our lab houses a fully motorized Leica DMi8 inverted fluorescence microscope with:
    • 5x, 10x, 20x, 40x, 63x & 100x PLAN objectives
    • Brightfield, differential interference contrast & phase contrast optics
    • Auto-focus
    • DAPI, FITC, TRITC & Texas Red fluorescence channels
    • Programmable for time lapse, multi-point or area scan experiments
    • Deconvolution and 3D projection of z-stacks

Wiggle