FCCF - Flow Cytometry Core Facility

Overview:

The Newcastle University FCCF delivers an innovative, cutting-edge cytometry provision to numerous research groups both at Newcastle University as well as national and international collaborators. It facilitates research within numerous disciplines including haematology, immunology, stem cell biology, developmental biology, bacterial studies, yeast studies and even synthetic engineered particles. The head of the FCCF is Dr Andrew Filby who has over 15 years' experience in cytometry-related research and associated technologies. He is also recognised by the International Society for the Advancement of Cytometry (ISAC) as a Shared Resource Laboratory (SRL) "Emerging Leader", one of only a handful worldwide to hold this accolade.

Facility location and composition:

In order to meet the needs of its extensive user base, the FCCF is currently located over two physical locations. The main facility hub is located at the Newcastle University Medical school and there is a satellite facility at International Centre for Life (iCFL). Across both locations there is a cutting edge analytical and cell sorting provision. This includes two multi-laser, multi-parameter Becton Dickinson FACS-Aria family cell sorters capable of sorting 4 populations simultaneously as well as a Beckman Coulter Astrios cell sorter that can sort up to six populations from a single sample. All sorters are located in biological containment hoods and are also capable of performing accurate and precise single cell deposition for onward genomic/transcriptomic analysis at the single cell level. At the medical school facility there is also a CyTOF Helios mass cytometer system that significantly expands the number of measurements that can be made at the single cell level. There is also collaborative access to an ImageStream X MKII system that is located in the Northern Institute for cancer Research (NICR). The FCCF are experts in linking these different cytometry platforms together in order to provide a synergistic and comprehensive cytometry-based solution across numerous research themes.

FCCF relationships and workflows within NUSCU:

The FCCF has many workflow synergies to the other facilities within NUSCU. For example, we will work closely with the BIU to study the observed cellular phenotypes identified in disaggregated tissue within the spatial context of the original tissue sites. We will work closely with the HTS facility and the GCF as they will process the single cell sorting outputs for genomic and transcriptomic analysis at the single cell level. Finally, everything we do will require input from the BSU at the experimental planning stage as well as how we analyse the parameter-rich data from high dimensional cytometry technologies such as the CyTOF Helios.

Contact

Flow Cytometry Core facility
Room M2.107/M2.099
Second Floor William Leech Building
Newcastle University
Framlington Place
Newcastle upon Tyne
NE2 4HH
United Kingdom
Telephone: +44 (0) 191 241 8831
Email: fccf@ncl.ac.uk
Web: http://www.ncl.ac.uk/fccf/

Staff:


Facility Head: Dr Andrew Filby (Andrew.filby@ncl.ac.uk)
Cytometry Specialist: Dr David McDonald (david.mcdonald@ncl.ac.uk)
Cytometry Specialists: Mr Andrew Fuller (Andrew.fuller@ncl.ac.uk)
Cytometry Technician: Dr Gillian Hulme (Gillian.hulme@ncl.ac.uk)
Cytometry Technician: Ms Carly Foster (carly.foster@ncl.ac.uk)
Cytometry Technician: Mr Jack Wigham (jack.wigham@ncl.ac.uk)

Astrios Helios