EM - EM Research Services
EM Research Services provide high resolution electron microscopy to students and researchers throughout Newcastle University, other academic institutions and commercial companies. We have both transmission and scanning electron microscopes as well as the cutting-edge technology of serial block face scanning EM. The facility is run by 3 experienced staff who can provide a full sample preparation service, training and image analysis.
Transmission electron microscopy
The facility houses a 100kV Philips CM100 TEM. Typical applications for TEM include tissue, cell culture (cell suspension and monolayers), isolated proteins, bacteria and viruses. For single cell analysis, cells are embedded in resin for ultrathin sectioning allowing visualisation of cell ultrastructure.
Scanning electron microscopy
The facility houses a Tescan VEGA LMU SEM. Typical applications for SEM include tissue, cell monolayers and bacteria. For single cell analysis, cells are dehydrated and gold-coated allowing visualisation of surface features.
Serial block face scanning electron microscopy
The facility houses a Zeiss Sigma FEG SEM incorporating the Gatan 3view system. Typical applications for SBFSEM include tissue, cell culture (cell suspension and monolayers) and bacteria. For single cell analysis, cells are embedded in resin and serially sectioned to generate a stack of images through the cell, which can be segmented to obtain 3D reconstructions at the ultrastructural level. Image analysis software available includes Amira and MIB.
EM Research Services Ground floor
Catherine Cookson Building Medical School
Newcastle upon Tyne.
Telephone: (0191) 208 6966